What type of Dynabead do I need? I wanted to talk a little about the selection characteristics of Agencourt’s AMPure beads, a bead-simple lab report combination that purifies PCR reactions. This stuff is incredible in terms of simplicity, efficiency, and high-throughput compatibility.
I have a sneaking suspicion that AMPure, not unlike fire to Prometheus, was handed down from the gods to benefit humanity. You just dunk it into your sample, slosh it around, stick it to a magnet, wash, wash again, and elute in your favorite buffer. We were wondering, though, about its selection process. What size fragments are selected by the AMPure beads, specifically at which ratio of beads to sample? The protocol recommends washing your sample in a 1. I added 30ul ladder to various concentrations of AMPure beads according to Agencourt’s instructions.